During this workshop, the Katharina Gaus Light Microscopy Facility will collaborate with Leica Microsystems to highlight the principles and applications associated with fluorescence lifetime–based imaging approaches. The focus will be on building both theoretical understanding and practical skills, including correct experimental setup, lifetime data acquisition, and analysis strategies. Participants will be introduced to methods such as decay fitting and phasor analysis, with applications spanning molecular interactions (e.g. distance measurements using FRET), cellular metabolism (e.g. NADH autofluorescence), and microenvironmental readouts such as pH and ion concentration.

Instruments, software, and analysis techniques covered:

• Leica STELLARIS 8 FALCON – confocal FLIM with fast lifetime acquisition and phasor analysis workflows

• Leica DIVE system – multiphoton FLIM for deep-tissue and intravital imaging

• PicoQuant FLIM/STED modules – time-domain FLIM and super-resolution approaches

• Leica LAS X software – lifetime acquisition and analysis environment

• Phasor analysis workflows – intuitive lifetime data interpretation

• Decay fitting approaches – model-based analysis for quantitative FLIM

• FRET applications – molecular distance measurements in live cells

• NADH autofluorescence imaging – probing cellular metabolism

Convenor Taryn Guinan, Leica Microsystems and Alex MacMillan, KGLMF

Duration Full day

Experience Level Suits all levels

Pricing $50.00

Please register for workshops by selecting them as optional add-ons via the LMA2026 registration StickyTickets website linked below.